lysenin his Search Results


lysenin his  (TargetMol)
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TargetMol lysenin his
OxLDL increases ASMase expression and promotes sphingomyelin (SM) catabolism. A , schematic illustration of SM catabolism. SM is hydrolyzed by ASMase to generate ceramide and phosphatidylcholine. Ceramidases convert ceramides into sphingosine and fatty acids. B , Western blot detection of ASMase expression is performed in cells treated with varying concentrations of oxLDL (50–120 μg/ml; one-way ANOVA followed by Tukey’s post hoc test, n = 3). C , RT-qPCR analysis measures ASMase mRNA levels in oxLDL (50 μg/ml) treated macrophages. D , ASMase enzymatic activity is assessed in cells treated with oxLDL at concentration of 50 μg/ml. E , OxLDL-treated cells are seeded on coverslip and incubated with SM-binding <t>protein,</t> <t>lysenin-His</t> (1 μg/ml). Membrane SM is detected using immunofluorescence staining and quantified by flow cytometry. F – H , total cellular levels of SM and ceramides, and SM/ceramide ratio are measured by LC-MS/MS in oxLDL-treated cells (50 μg/ml). I and J , lipidomics analysis of specific SM and ceramide species in cells treated with oxLDL (50 μg/ml). All comparisons were conducted with Student's t test except in ( B ) (mean ± SD, n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.
Lysenin His, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysenin+his/Lysenin+Protein%2C+Eisenia+fetida%2C+Recombinant/pmc12180984-321-7-8
Average 93 stars, based on 1 article reviews
lysenin his - by Bioz Stars, 2026-10
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OxLDL increases ASMase expression and promotes sphingomyelin (SM) catabolism. A , schematic illustration of SM catabolism. SM is hydrolyzed by ASMase to generate ceramide and phosphatidylcholine. Ceramidases convert ceramides into sphingosine and fatty acids. B , Western blot detection of ASMase expression is performed in cells treated with varying concentrations of oxLDL (50–120 μg/ml; one-way ANOVA followed by Tukey’s post hoc test, n = 3). C , RT-qPCR analysis measures ASMase mRNA levels in oxLDL (50 μg/ml) treated macrophages. D , ASMase enzymatic activity is assessed in cells treated with oxLDL at concentration of 50 μg/ml. E , OxLDL-treated cells are seeded on coverslip and incubated with SM-binding protein, lysenin-His (1 μg/ml). Membrane SM is detected using immunofluorescence staining and quantified by flow cytometry. F – H , total cellular levels of SM and ceramides, and SM/ceramide ratio are measured by LC-MS/MS in oxLDL-treated cells (50 μg/ml). I and J , lipidomics analysis of specific SM and ceramide species in cells treated with oxLDL (50 μg/ml). All comparisons were conducted with Student's t test except in ( B ) (mean ± SD, n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.

Journal: The Journal of Biological Chemistry

Article Title: Acid sphingomyelinase recruits palmitoylated CD36 to membrane rafts and enhances lipid uptake

doi: 10.1016/j.jbc.2025.110213

Figure Lengend Snippet: OxLDL increases ASMase expression and promotes sphingomyelin (SM) catabolism. A , schematic illustration of SM catabolism. SM is hydrolyzed by ASMase to generate ceramide and phosphatidylcholine. Ceramidases convert ceramides into sphingosine and fatty acids. B , Western blot detection of ASMase expression is performed in cells treated with varying concentrations of oxLDL (50–120 μg/ml; one-way ANOVA followed by Tukey’s post hoc test, n = 3). C , RT-qPCR analysis measures ASMase mRNA levels in oxLDL (50 μg/ml) treated macrophages. D , ASMase enzymatic activity is assessed in cells treated with oxLDL at concentration of 50 μg/ml. E , OxLDL-treated cells are seeded on coverslip and incubated with SM-binding protein, lysenin-His (1 μg/ml). Membrane SM is detected using immunofluorescence staining and quantified by flow cytometry. F – H , total cellular levels of SM and ceramides, and SM/ceramide ratio are measured by LC-MS/MS in oxLDL-treated cells (50 μg/ml). I and J , lipidomics analysis of specific SM and ceramide species in cells treated with oxLDL (50 μg/ml). All comparisons were conducted with Student's t test except in ( B ) (mean ± SD, n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.

Article Snippet: After blocking with 2% BSA/PBS, 1 μg/ml lysenin-His (TargetMol) dissolved in the blocking solution was added and incubated at 4 °C overnight.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Activity Assay, Concentration Assay, Incubation, Binding Assay, Membrane, Immunofluorescence, Staining, Flow Cytometry, Liquid Chromatography with Mass Spectroscopy