lysenin his Search Results


lysenin his  (TargetMol)
93
TargetMol lysenin his
OxLDL increases ASMase expression and promotes sphingomyelin (SM) catabolism. A , schematic illustration of SM catabolism. SM is hydrolyzed by ASMase to generate ceramide and phosphatidylcholine. Ceramidases convert ceramides into sphingosine and fatty acids. B , Western blot detection of ASMase expression is performed in cells treated with varying concentrations of oxLDL (50–120 μg/ml; one-way ANOVA followed by Tukey’s post hoc test, n = 3). C , RT-qPCR analysis measures ASMase mRNA levels in oxLDL (50 μg/ml) treated macrophages. D , ASMase enzymatic activity is assessed in cells treated with oxLDL at concentration of 50 μg/ml. E , OxLDL-treated cells are seeded on coverslip and incubated with SM-binding <t>protein,</t> <t>lysenin-His</t> (1 μg/ml). Membrane SM is detected using immunofluorescence staining and quantified by flow cytometry. F – H , total cellular levels of SM and ceramides, and SM/ceramide ratio are measured by LC-MS/MS in oxLDL-treated cells (50 μg/ml). I and J , lipidomics analysis of specific SM and ceramide species in cells treated with oxLDL (50 μg/ml). All comparisons were conducted with Student's t test except in ( B ) (mean ± SD, n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.
Lysenin His, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysenin his/product/TargetMol
Average 93 stars, based on 1 article reviews
lysenin his - by Bioz Stars, 2026-04
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lysenin protein  (Biosynth Carbosynth)
86
Biosynth Carbosynth lysenin protein
In some biopsies, SM is present in sinusoidal Kupffer cells only (K distribution). In other biopsies, SM has accumulated in sinusoidal Kupffer cells, foci of proliferating portal Kupffer cells and hepatocytes (KH distribution). A. Patient 6: SM is present within enlarged, foamy Kupffer cells (arrows; H&E, 60× objective). B. Patient 1: SM is present within clusters of enlarged foamy Kupffer cells (K) and foamy hepatocytes (H) (H&E, 60× objective). C. In HRLM sections, SM stains purple in sinusoidal Kupffer cells (modified toluidine blue, 100× oil objective). D. In KH distribution, SM is present in both Kupffer cells and hepatocytes (modified toluidine blue, 100× oil objective). E and F. <t>Lysenin</t> <t>affinity</t> <t>staining</t> also highlights the differences in SM accumulation in K versus KH distribution patterns (lysenin affinity stain, 100× oil objective). G. CD68 immunohistochemistry highlights the enlarged Kupffer cells as single cells (10× objective). H. CD68 immunohistochemistry highlights enlarged Kupffer cells as single cells and as clusters proliferating in portal tracts and infiltrating bands of fibrosis (10× objective).
Lysenin Protein, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysenin protein/product/Biosynth Carbosynth
Average 86 stars, based on 1 article reviews
lysenin protein - by Bioz Stars, 2026-04
86/100 stars
  Buy from Supplier

Image Search Results


OxLDL increases ASMase expression and promotes sphingomyelin (SM) catabolism. A , schematic illustration of SM catabolism. SM is hydrolyzed by ASMase to generate ceramide and phosphatidylcholine. Ceramidases convert ceramides into sphingosine and fatty acids. B , Western blot detection of ASMase expression is performed in cells treated with varying concentrations of oxLDL (50–120 μg/ml; one-way ANOVA followed by Tukey’s post hoc test, n = 3). C , RT-qPCR analysis measures ASMase mRNA levels in oxLDL (50 μg/ml) treated macrophages. D , ASMase enzymatic activity is assessed in cells treated with oxLDL at concentration of 50 μg/ml. E , OxLDL-treated cells are seeded on coverslip and incubated with SM-binding protein, lysenin-His (1 μg/ml). Membrane SM is detected using immunofluorescence staining and quantified by flow cytometry. F – H , total cellular levels of SM and ceramides, and SM/ceramide ratio are measured by LC-MS/MS in oxLDL-treated cells (50 μg/ml). I and J , lipidomics analysis of specific SM and ceramide species in cells treated with oxLDL (50 μg/ml). All comparisons were conducted with Student's t test except in ( B ) (mean ± SD, n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.

Journal: The Journal of Biological Chemistry

Article Title: Acid sphingomyelinase recruits palmitoylated CD36 to membrane rafts and enhances lipid uptake

doi: 10.1016/j.jbc.2025.110213

Figure Lengend Snippet: OxLDL increases ASMase expression and promotes sphingomyelin (SM) catabolism. A , schematic illustration of SM catabolism. SM is hydrolyzed by ASMase to generate ceramide and phosphatidylcholine. Ceramidases convert ceramides into sphingosine and fatty acids. B , Western blot detection of ASMase expression is performed in cells treated with varying concentrations of oxLDL (50–120 μg/ml; one-way ANOVA followed by Tukey’s post hoc test, n = 3). C , RT-qPCR analysis measures ASMase mRNA levels in oxLDL (50 μg/ml) treated macrophages. D , ASMase enzymatic activity is assessed in cells treated with oxLDL at concentration of 50 μg/ml. E , OxLDL-treated cells are seeded on coverslip and incubated with SM-binding protein, lysenin-His (1 μg/ml). Membrane SM is detected using immunofluorescence staining and quantified by flow cytometry. F – H , total cellular levels of SM and ceramides, and SM/ceramide ratio are measured by LC-MS/MS in oxLDL-treated cells (50 μg/ml). I and J , lipidomics analysis of specific SM and ceramide species in cells treated with oxLDL (50 μg/ml). All comparisons were conducted with Student's t test except in ( B ) (mean ± SD, n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.

Article Snippet: After blocking with 2% BSA/PBS, 1 μg/ml lysenin-His (TargetMol) dissolved in the blocking solution was added and incubated at 4 °C overnight.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Activity Assay, Concentration Assay, Incubation, Binding Assay, Membrane, Immunofluorescence, Staining, Flow Cytometry, Liquid Chromatography with Mass Spectroscopy

In some biopsies, SM is present in sinusoidal Kupffer cells only (K distribution). In other biopsies, SM has accumulated in sinusoidal Kupffer cells, foci of proliferating portal Kupffer cells and hepatocytes (KH distribution). A. Patient 6: SM is present within enlarged, foamy Kupffer cells (arrows; H&E, 60× objective). B. Patient 1: SM is present within clusters of enlarged foamy Kupffer cells (K) and foamy hepatocytes (H) (H&E, 60× objective). C. In HRLM sections, SM stains purple in sinusoidal Kupffer cells (modified toluidine blue, 100× oil objective). D. In KH distribution, SM is present in both Kupffer cells and hepatocytes (modified toluidine blue, 100× oil objective). E and F. Lysenin affinity staining also highlights the differences in SM accumulation in K versus KH distribution patterns (lysenin affinity stain, 100× oil objective). G. CD68 immunohistochemistry highlights the enlarged Kupffer cells as single cells (10× objective). H. CD68 immunohistochemistry highlights enlarged Kupffer cells as single cells and as clusters proliferating in portal tracts and infiltrating bands of fibrosis (10× objective).

Journal: The American Journal of Surgical Pathology

Article Title: Liver and Skin Histopathology in Adults with Acid Sphingomyelinase Deficiency (Niemann-Pick Disease Type B)

doi: 10.1097/PAS.0b013e31825793ff

Figure Lengend Snippet: In some biopsies, SM is present in sinusoidal Kupffer cells only (K distribution). In other biopsies, SM has accumulated in sinusoidal Kupffer cells, foci of proliferating portal Kupffer cells and hepatocytes (KH distribution). A. Patient 6: SM is present within enlarged, foamy Kupffer cells (arrows; H&E, 60× objective). B. Patient 1: SM is present within clusters of enlarged foamy Kupffer cells (K) and foamy hepatocytes (H) (H&E, 60× objective). C. In HRLM sections, SM stains purple in sinusoidal Kupffer cells (modified toluidine blue, 100× oil objective). D. In KH distribution, SM is present in both Kupffer cells and hepatocytes (modified toluidine blue, 100× oil objective). E and F. Lysenin affinity staining also highlights the differences in SM accumulation in K versus KH distribution patterns (lysenin affinity stain, 100× oil objective). G. CD68 immunohistochemistry highlights the enlarged Kupffer cells as single cells (10× objective). H. CD68 immunohistochemistry highlights enlarged Kupffer cells as single cells and as clusters proliferating in portal tracts and infiltrating bands of fibrosis (10× objective).

Article Snippet: For SM staining, a 5μg/mL solution of lysenin protein (Peptides International; Louisville, KY) was diluted in TBS with 0.5% BSA (TBS-B, DAKO) and placed on the slides and incubated at 37°C for 1 hour.

Techniques: Modification, Staining, Immunohistochemistry